Un protoplasto es una célula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . El poder seleccionar eficientemente las plantas híbridas producidas a partir de fusión de protoplastos es fundamental. Se han logrado muchos avances en el.

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Protoplasts fusion from S. None of the regenerants had 72 chromosomes, which results from the protoplasts fusion of a tetraploid with a diploid genome. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L.

The numbers of chromosome of S. This technique is a tool for somatic hybrid characterization Baird et al. Interspecific fuison hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L.

In this study, confirmation of fusion-derived regenerants by the use of RAPD markers represented an effective system for the detection of somatic hybrids from S. Final concentration was protoplasts per millilitre.

Fusion de Protoplastos by Arely Gutierrez on Prezi

These samples were obtained from rooted plants maintained in a greenhouse. Potato, Solanum tuberosum L. The hybridity of the fusion regenerants was confirmed by RAPD markers. Instpp After that, fuusion settled down. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. This technique has created novel cellular genome configurations by combining sexually incongruent species.


The latter has a natural resistance to late blight, Phytophthora infestans Mont. Gels were analyzed and photographed under UV light. The morphology of fusion-derived regenerants was intermediate between the donor parents. In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S. Tissue culture procedures to produce somatic hybrids and the application df molecular biological protoplaatos for their analysis are well established.

Fusion de protoplastos pdf

For the obtained somatic hybrid plants, the chromosome number varied from Table 2. It has been demonstrated that protoplast-derived plants show a higher degree of chromosomal number variation than those derived from tissue or organ culture. Please sign in or create an account. Get cutting-edge science videos from J o VE sent straight to your inbox every month.

Chromosome observations indicated that all hybrids were aneuploids. Transfer of resistance to potato leaf roll virus from Solanum protoplastoa into Solanum tuberosum by somatic fusion. Protoplasts of both types are mixed in an equal proportion attaining a density of 5 x 10 4 to 2 x 10 5 protoplasts per ml.


Click here for the english version. Prior to fusion, protoplasts from S. Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusion from S. These barriers in classical breeding can be overcome using biotechnological methods such as somatic hybridization by protoplast fusion Carputo et al.

Analysis of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and Solanum sanctae-rosae. Resistance to Phytophthora infestans in Solanum tuberosum and wild Solanum species. Plant material and protoplast isolation. Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome.

Introduction Potato, Solanum tuberosum L. Shoots also showed purple-colored meristematic zones. Under these conditions, calli hybrids follow the developmental pathway of the morphogenic parents. Materials and methods Plant material and protoplast isolation.