Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method
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AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but is cumbersome and time consuming for routine quality control and screening tests. The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings.
A 50 TM Copyright? Your comments will receive careful consideration at a meeting of the responsible technical committee, which you may attend. The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings.
Antimicrobial – Parallel Streak Method AATCC 147
This test method is subject to revision at any time by the responsible technical committee and must be reviewed every five years and if not revised, either reaffirmed or withdrawn. At the conclusion of two weeks, make a fresh transplant from stock culture.
In the Parallel Streak Method, the agar surface is inoculated making it easier to distinguish between the test organism and contaminant organisms which may be present on the unsterilized specimen.
A standard strain of bacteria is used which is specific to the requirements of the material under test. Notes and References Individual reprints single or multiple copies of this method may be obtained by contacting AATCC at the above address or tel: Foreword The Parallel Streak Method has filled a need for a relatively quick and easily executed qualitative method to determine antibacterial activity of diffusable antimicrobial agents on treated textile materials.
This is not necessary if prepared, dehydrated medium is used. In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise. Other recommended strains are listed below in Section 6. Users of this test method are expressly advised that determination of the validity of any such patent rights, and the risk of infringement of such rights, are entirely their own responsibility.
The method is useful for obtaining a rough estimate of activity in that the growth of the inoculum organism decreases from one end of each streak to the other and from one streak to the next resulting in increasing degrees of sensitivity.
Safety Precautions reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment. Culture Medium mm length permits the specimens to lie across 5 parallel inoculum streaks each of diminishing width from about 8 mm to 4 mm wide.
Maintenance of Culture of Test Organisms 8. AATCC grants you a license as follows: Your comments are invited either for merhod of this test method or for additional methods and should be addressed to the AATCC Technical Center. The Parallel Streak Method has proven effective over a number of years of use in providing evidence of antibacterial activity against both Gram positive and Gram negative bacteria.
AATCC takes no position respecting the validity of any patent rights asserted in connection with any item mentioned in this test method. The results of using this procedure have been demonstrated by Committee RA31 to be reproducible methpd various laboratories working with materials containing residual amounts of antibacterial agents as determined by chemical assay after multiple standard washings.
Wear safety glasses in all laboratory areas. Many standard textile finishing chemicals, especially crease resistant and permanent press reagents, will often give strong antibacterial activity even after many washes.
Allow agar atcc gel firmly before inoculating. Check pH and adjust to 7. Avoid contamination by using good sterile technique in plating and transferring.
The hard copy print may only be distributed to other employees for their internal use within your organization. Check culture purity by making streak plates periodically and observing for a single species-characteristic type of colonies.
AATCC 147 – Antimicrobial Fabric Test
If no other bacterial species is specified, Staphylococcus aureus may be used as a representative Gram positive organism. The test Method determines antibacterial activity of diffusable antimicrobial agents on treated textile materials.
If you feel that your comments have not received a fair hearing, you should make your views known to Christopher S. Take care not to break the surface of the agar while making the streaks. Department of Health and Human services publication Biosafety in Microbiological and Biomedical Laboratories should be consulted see Until a precision statement is generated for this test method, use standard statistical techniques in making any comparisons of test results for either within-laboratory or between-laboratory averages.
These safety precautions are for information purposes only. Parallel Streak Method Developed in by AATCC Committee RA31; reaffirmed, ; editorially revised,; revisedwith title change; editorially revised and reaffirmed Antibacterial Activity Assessment of Textile Materials: A zone of inhibition occurs as a result of the diffusion of an antimicrobial agent from the specimen.
After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. The precautions are ancillary to the testing procedures and are not intended to be all inclusive. Adjust to pH 6. Wear protective clothing and respiratory protection that prevents penetration by the bacteria.
Report of results will include an observation of zones of inhibition and growth under the specimen if present.
kethod BoxManassas VA ; tel: After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. To constitute acceptable antibacterial activity, there must be no bacterial colonies directly under the sample in the contact area.
Other organisms can also be used depending on the intended end-use.
AATCC – Antimicrobial Fabric Test | Situ Biosciences LLC
Neither the electronic file nor the hard copy print may be reproduced in any way. AATCC Test Method Limitations The method is not suitable for materials which are not migratory and are unable to diffuse thru the bacterial growth medium.
The average width of a zone of inhibition along a streak on either side of the test specimen may be calculated using the following equation: Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7.
Leonard, Technical Director, at the address shown below.